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1.) Using proteomics to quantitate and characterized cell membranes, subcellular organelles, and cell-cycle regulation.

2.) Absolute Quantification (AQua). Heavy isotope-labeled tryptic peptides can be used as ideal internal standrads to determine absolute quantities of proteins by tandem mass spectrometry (Gerber et al., PNAS 2003). We are interested in applying this technique to gain quantitative understanding of specific cell signaling events. We are currently quantifying the multi-site phosphorylation status of Cdc2 directly from whole cell extracts.

3a.) Developing methods which may will allow us to determine if proteins within complex mixtures can be identified by accurate mass alone
3b.) Automating data analysis and presentation.

4.) Lipidomics: Characterizing lipid vesicles and their roles & functions in the cell.