Lab Methods
Our main approach is to introduce genetic modifications into the mouse genome, analyze the phenotypic consequences in vascular development and deduce possible mechanisms underlying developmental defects. We maintain a within-the-lab ES cell facility and construct mouse embryos directly from modified ES cells by tetraploid as well as diploid aggregation. This approach often allows us to by-pass the need of germline transmission.
Other methods employed in our research include immunohistochemistry and imaging techniques such as confocal microscopy. In addition, we employ recent molecular biology tools such as RNA interference and BAC cloning to speed up the process of modifying gene expression in ES cells or mouse embryos.
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