David K. Han, Ph.D.
Center for Vascular Biology
University of Connecticut Health Center
263 Farmington Avenue
Farmington, CT 06030-3501
|David Han, Ph.D.
Member, Center for Vascular Biology
Associate Professor of Cell Biology
The overall goal of the Han Laboratory is to utilize
newly developed proteomic technologies to uncover cellular
signaling networks and pathways that govern mechanisms of
programmed cell death/apoptosis. Toward the overall goal,
we have made significant level of commitment to improve
currently available technologies and apply proteomic
technologies to uncover novel biological insights.
- Apoptotic Signaling Networks in Vascular Inflammation
- Mechanisms of Apoptotic Cell Engulfment
- Modeling Genomics and Proteomics Datasets by Developing
Novel Bioinformatics Approaches
- Implementation of Proteomics and Mass Spectrometry
Technologies for Protein Identification and Quantification
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- Using proteomics to quantitate and characterized cell
membranes, subcellular organelles, and cell-cycle
- Absolute Quantification (AQua). Heavy isotope-labeled
tryptic peptides can be used as ideal internal standards to
determine absolute quantities of proteins by tandem mass
spectrometry (Gerber et al., PNAS 2003). We are interested
in applying this technique to gain quantitative
understanding of specific cell signaling events. We are
currently quantifying the multi-site phosphorylation status
of Cdc2 directly from whole cell extracts.
- Developing methods which will allow us to determine
if proteins within complex mixtures can be identified by
accurate mass alone.
- Automating data analysis and presentation.
- Lipidomics: Characterizing lipid vesicles and their
roles & functions in the cell.
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